Unit V: Protein and Gene Manipulation (40 Marks)
Chapter I: Recombinant DNA Technology
Introduction; Tool of rDNA
Technology; Marketing Recombinant DNA; Introduction of Recombinant DNA
into Host Cells; Identification of Recombinants; DNA Library; DNA
Probes; Hybridization Techniques; Polymerase Chain Reaction (PCR); DNA
Secquencing; Site-directed Mutagenesis
Chapter II: Genomics and Bioinformatics
Introduction; Genome Sequencing
Projects; Gene Prediction and Counting; Genome Similarity, SNPs and
Comparative Genomics; Functional Genomics; Proteomics; History of
Bioinformatics; Sequences and Nomenclature; Information
Sources; Analysis using Bioinformatics Tools
Chapter III: Proteing Structure and Engineering
Introduction to the World of Proteins; 3-D Shape of Proteins; Structure-Function Relationship in Proteins
Purification of Proteins; Characterization of Proteins; Protein based Products; Designing Proteins
Purification of Proteins; Characterization of Proteins; Protein based Products; Designing Proteins
Unit VI: Cell Culture Technology (30 Marks)
Chapter I: Microbial Culture and Applications
Introduction; Microbial Culture
Techniques; Measurement and Kinetics of microbial Growth; Scale up of
microbial process; Isolation of microbial products; Strain isolation and
Improvement; Applications of microbial culture technology; Biosafety of
genetically modified microbes
Chapter II: Plant Cell Culture and Applications
Introduction; Cell and Tissue Culture
Techniques; Applications of Cell and Tissue Culture; Gene Transfer
Methods in Plants; Transgenic Plants with Beneficial Traits; Biosafety
in Plant Genetic Engineering
Chapter III: Animal Cell Culture and Applications
Introduction; Animal Cell Culture
Techniques; Characterisation of Cell Lines; Scale-up of Animal Culture
Process; Applications of Animal Cell Culture; Stem Cell Technology; Bio
safety of genetically modified animals
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